![]() The construct was then subcloned into a pET28a vector (Novagen). 6 The construct was designed by adding BamH1 and Xho1 restriction sites at the N‐terminus and C‐terminus, respectively. 100031579, Integrated DNA Technologies) consisting of 417 oligonucleotide bases that was optimized according to our previous work. Molecular cloning and development of recombinant hTSHR‐169 proteinĬonstruct of hTSHR‐169 cDNA was synthetically made through GBlock gene fragments (Ref. Herein, we compared ELISA and dot blot of TSH‐receptor antibody tests for their ability to diagnose GD.Ģ.1. However, this particular recombinant’s utility for GD diagnosis has not been assessed. ![]() 1, 4 Therefore, in this study, we developed a recombinant protein of hTSHR‐169, which was specifically recognized TRAb from the serum of GD patients, in order to generate lateral flow‐based immunoassay for diagnosing GD case. However, the sensitivity and specificity of TRAb assay are relatively comparable, ranging from 79.5 to 94.4% and 87.5 to 97.9%, respectively. The diagnostic performance of TRAb for GD differs according to the TRAb detection method. Hence, studies examining TRAb levels during the initial diagnosis of GD are relatively scarce. 1, 3, 5Īlthough TRAb is easy to perform, the measurement of TRAb levels is not routinely employed in all suspected GD patients. Additionally, the role of TRAb is not only in confirming GD diagnosis but also potential in predicting the clinical course of GD, relapse risk, and treatment responses. 1, 3 It is now well established that thyroid‐stimulating hormone receptor autoantibody (TRAb) is the serological hallmark of GD, 4 which is usually helpful in differentiating GD from other causes of hyperthyroidism. 1, 2 Clinically, GD is characterized by the suppression of TSH levels, overstimulation of thyroid hormones, and the production of antithyroid antibodies. Graves’ disease (GD) is a chronic autoimmune disorder of the thyroid gland, affecting nearly 0.5% of the general population, with a higher incidence among females relative to males.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |